Label-free and fluorescent imaging data of microparticle association with Salmon Keratocytes

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Thiyagarajan, Dhivya B; Afzal, Bilal M; Wolfson, Deanna, 2024, "Label-free and fluorescent imaging data of microparticle association with Salmon Keratocytes", https://doi.org/10.18710/KBEQT9, DataverseNO, V1

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Description	The dataset contains multimodal and multidimensional microscopy images of the interactions between different microparticles either in skin keratocytes cells (SKC) or in corneal keratocyte cells (CKC) of Atlantic Salmon. All images were captured at room temperature, and the dataset includes both test (with microparticles) and control (without microparticles) conditions. These interactions were captured using two different microscopes: 1) 3D Cell Explorer-fluo (Nanolive): a holotomographic imaging method which produces label-free 3D images using refractive index as the contrast mechanism and can optionally also provide 2D correlative fluorescence images. 2) DeltaVision Elite (GE Healthcare): a widefield fluorescence microscope with integrated deconvolution for improved 3D imaging, which can provide correlative 2D differential interference contrast (DIC) images. The main goal of this study was to understand how different microparticles interact with SKC and CKC, which are two of the important defense cells for innate immunity in fish. This dataset may be useful for researchers interested in studying salmon skin and corneal cell behavior, those who are interested in cell-microparticle interactions, as well as those interested in accessing or processing a relatively new type of microscopy image (holotomography). (2024-03-25)
Subject	Medicine, Health and Life Sciences
Keyword	Salmon, Keratocyte, Microscopy, Holotomography, Microplastics, Fluorescence
License/Data Use Agreement	CC0 1.0

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Citation Metadata

Persistent Identifier	doi:10.18710/KBEQT9
Publication Date	2024-06-28
Title	Label-free and fluorescent imaging data of microparticle association with Salmon Keratocytes
Author	Thiyagarajan, Dhivya B (UiT The Arctic University of Norway) - ORCID: 0000-0001-5172-8163 Afzal, Bilal M (UiT The Arctic University of Norway) Wolfson, Deanna (UiT The Arctic University of Norway) - ORCID: 0000-0001-6059-2472
Point of Contact	Use email button above to contact. Wolfson, Deanna (UiT The Arctic University of Norway)
Description	The dataset contains multimodal and multidimensional microscopy images of the interactions between different microparticles either in skin keratocytes cells (SKC) or in corneal keratocyte cells (CKC) of Atlantic Salmon. All images were captured at room temperature, and the dataset includes both test (with microparticles) and control (without microparticles) conditions. These interactions were captured using two different microscopes: 1) 3D Cell Explorer-fluo (Nanolive): a holotomographic imaging method which produces label-free 3D images using refractive index as the contrast mechanism and can optionally also provide 2D correlative fluorescence images. 2) DeltaVision Elite (GE Healthcare): a widefield fluorescence microscope with integrated deconvolution for improved 3D imaging, which can provide correlative 2D differential interference contrast (DIC) images. The main goal of this study was to understand how different microparticles interact with SKC and CKC, which are two of the important defense cells for innate immunity in fish. This dataset may be useful for researchers interested in studying salmon skin and corneal cell behavior, those who are interested in cell-microparticle interactions, as well as those interested in accessing or processing a relatively new type of microscopy image (holotomography). (2024-03-25)
Subject	Medicine, Health and Life Sciences
Keyword	Salmon Keratocyte Microscopy Holotomography Microplastics Fluorescence
Language	English
Producer	UiT The Arctic University of Norway (UiT) https://en.uit.no/
Contributor	Hosting Institution : Norwegian College of Fishery Science Hosting Institution : Institute of Physics and Technology Data Collector : Sette, Marie HS Project Leader : Dalmo, Roy Work Package Leader : Ahluwalia, Singh Balpreet Funder : Agarwal, Krishna
Funding Information	The Research Council of Norway: 301401 UiT The Arctic University of Norway: Tematisk Satsinger 2061348
Distributor	UiT The Arctic University of Norway (UiT) https://dataverse.no/dataverse/uit
Depositor	Thiyagarajan, Dhivya Borra
Deposit Date	2024-03-25
Date of Collection	Start Date: 2020-09-01 ; End Date: 2023-12-28
Data Type	experimental data
Series	Holotomography Deconvoluted
Software	Fiji/limageJ, Version: https://imagej.net/ij/features.html steve, Version: https://www.nanolive.ch/products/3d-microscopes/cx/
Related Dataset	Opstad, Ida S.; Wolfson, Deanna L., 2021, "Salmon keratocyte DIC microscopy videos", https://doi.org/10.18710/Q57B06, DataverseNO, V1

Geospatial Metadata

Geographic Coverage	Norway, Troms, Tromsø

Life Sciences Metadata

Other Design Type	Microparticle interactions using label-free microscopy
Factor Type	Cell Type/Cell Line
Other Organism	Atlantic salmon (salmo salar)
Measurement Type	Other
Other Technology Platform	Nanolive; Deltavision microscopy
Cell Type	Salmon Keratocyte Cells

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